Sample information
Picture	Photos by: Cedar M, Ben M, Naiya W
Location
Collection date
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations	In a garage
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system Bio-Rad
Buffer	0.25 TAE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	new student
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Arthropoda was found to be negative for Wolbachia.