Wolf Spider (Trochosa ruricola)

April 1, 2025

Sample information
Picture
Photos by: Brittanie T.
Location
Collection date 08/14/2024
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
ATAGTGGGGACTGCTATAAGAGTGTTAATTCGTTTAGAATTAGGAAATTCTGGAAGTTTATTAGGAGATGATCATTTATATAATGTTATAGTTACTGCTCATGCTTTTGTGATGATTTTTTTTATAGTAATGCCAATTCTTATTGGAGGTTTTGGTAATTGATTAATTCCTTTAATGTTAGGTGCTCCTGATATATNNTTTCCTCGAATGAATAATCTTTCTTTTTGGTTATTGCCCCCTTCTTTATTTTTATTAACTATGTCTTCTATAATGGAAATAGGGGTAGGAGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTANAATAGGTCATATAGGGAGTTCTATAGATTTTGCTATTTTTTCTCTTCATTTANCTGGTGCTTCTTCTATTATAGGGGCAGTAAATTTTATTTCTACTATTATTAATATACGAATATTAGGTATATCTATAGATAAGGTACCTTTATTTGTTTGGTCAGTTTTGATTACGGCTGTTTTATTATTATTATCTTTACCTGTTTTAGCAGGTGCTATTACTATGTTATTAACTGATCAAAATTTTAATACTTCTTTTTTTGACCCTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
Report Inappropriate Post