Isopoda (Woodlouse) 3C1

May 6, 2025

Sample information
Picture
Photos by: By Elisabet C.
Location
Collection date 03/09/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Brown pillbug with pale yellow-beige accents caught in an urban forest during spring.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Trachelipodidae Trachelipus Trachelipus rathkii

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

It was shown in the CO1 gel electrophoresis that the sample did not contain the CO1 gene. This is most likely due to an error during DNA extraction, which means that the absence of Wolbachia also came from this error.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Trachelipus rathkii was found to be negative for Wolbachia.
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