Armadillidium vulgare

April 2, 2025

Sample information
Picture
Photos by: Kody H., Pranav D.
Location
Collection date 03/11/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

The pill bug was found near the stream’s bend.

Although the habitat was near the stream, pillbugs live on the land. It was found near the bed of the stream, underneath some of the rocks, near where it was dirty and the rock left a sort of dent in the groun.d.

The pillbug appears to be somewhat rolled up, but not completely stretched out. Also, it looks to be black and brown, and its legs are curled up.

Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

First, the negative control showed up, which could mean some error in our protocol or materials. Also, in the co1 gel, my sample’s band (sampleB6), which checked if the DNA was from an arthropod, did not show up, leading to my low confidence level, as other results would be inconclusive if my sample did indeed not include arthropod DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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