Water Strider

April 2, 2025

Sample information
Picture
Photos by: Samhita K.
Location
Collection date 03/12/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

It was collected on the Indian Run Stream bend, floating on the water. Late winter, 52 degrees Fahrenheit, and my arthropod is big and black and has one long body with four legs in the back and two legs coming out in the front.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Gerridae Aquarius Aquarius najas

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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