Sample information
Picture	Photos by: Akshara K
Location
Collection date	03/12/2025
Captive / Cultivated?	Wild-caught
Group	Thomas Jefferson High School for Science and Technology
Observations	Collected in the water, brown in color, long body. Collected late winter, 52 degrees C.
Putative identification	Arthropoda Hexapoda Insecta Hemiptera Gerridae
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Body without legs
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary