Sample information
Picture
Location	No map location data available.
Collection date	03/12/2025
Captive / Cultivated?	Wild-caught
Group	Thomas Jefferson High School for Science and Technology
Observations
Putative identification	Arthropoda Hexapoda Insecta Diptera
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Diptera was found to be negative for Wolbachia.