Tapinoma sessile pd 7

April 7, 2025

Sample information
Picture
Photos by: Jacqueline A., Julia S.
Location
Collection date 03/09/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Tapinoma Tapinoma sessile

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was clearly a COI band for the A1 sample in our gel, showing that my ant (A1)’s DNA was successfully extracted. There is clearly not a band in my ant (A1)’s lane in the Wolbachia gel while other lanes did have strong bands, so the procedure did work for determining whether samples had Wolbachia. Thus, my ant did not have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
ATAAGAATAATTATTCGTATTGAATTAGGGACCTGCGGAGCTTTAATTAGTAATGATCAAATCTATAATTCAATTGTAACAGGACATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTAGAAGTGGGGGCTTTGGAAATTTTTTAGTCCCCTTAATACTCGGAGCCCCAGATATACCTTACCCCCGAATAAATAATATAAGATTTTGATTACTACCGCCCTCAATCCTTTTACTAACTATTAGAAATTTTATCAGATCTGGGGTAGGGACCGGATGAACAGTTTACCCGCCCCTAGCATCTAATATCTATCATAATGGACCTTCTGTAGATTTATCTATTTTCTCCCTTCATATTGCTGGTATATCCTCAATTTTAGGGGCAATTAATTTTATTTCTACTATTATTAATATACACCATAAAAATTTTTCCACAGATAAAATCCCTCTTTTAGTATGATCAATCTTAATTACTGCAATTTTATTACTCCTATCTCTCCCAGTTCTAGCTGGGGCTATTACTATACTTTTAACAGATCGAAACTTTAAATACATCCTTTTTTGACCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Tapinoma sessile was found to be negative for Wolbachia.
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