Sample information
Picture	Photos by: Anjana V.
Location
Collection date	03/25/2025
Captive / Cultivated?	Wild-caught
Group	Scientists in Residence
Observations	Found on Campus. Sunny day, but cold out. Alive
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera Formicidae
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Alive bug so I think I did well on the DNA extraction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Formicidae was found to be negative for Wolbachia.