DEMZJC

April 14, 2025

Sample information
Picture
Photos by: Zahra C
Location
Collection date 06/06/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Cold ground; cloudy and rainy weather
Small shrubs and tall, covered moss tree’s; cluttered piles of leaves & twig on the ground
Abandoned field with tree trunks covered in moss and vines by piles of sand and dirt

collected with gloved hands
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence level is high because I know that not many organisms contain Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
TAGTAGGGACATCTTTAAGAATATTAatctcgaGCAGAACTAAATctaCC TGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGGCTC ACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGAGGA TTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATT TCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTATCTC TTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGAACC GTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGTAGA TTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAGGTG CCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATAAGC CCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATTATT ATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTATTAA CAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGTGAT CCTATTTTATATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be negative for Wolbachia.
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