DEM-EMV Blattodea

April 14, 2025

Sample information
Picture
Photos by: Elizabet V
Location
Collection date 01/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Insect was observed to have true legs, mandibles, beaded antennae, and no wings. 
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The gel electrophoresis was uncontaminated and showed a negative result for my subject.
Wolbachia 16S sequence
Arthropod COI sequence
AATGGTCGGAACATCACTTAGAATACTAATTCGAACAGAACTAGGGctaC CTGGATCTCTAATTGGAGATGACCAGATCTACAACGTTATCGTTACTGCT CATGCATTCGTCATAATTTTCTTTATGGTCATACCAATCATGATTGGTGG ATTCGGAAACTGACTAGTACCACTTATATTAGGAGCCCCAGACATGGCAT TCCCACGAATAAACAACATAAGATTTTGATTACTCCCACCATCATTAACA TTACTACTTACTAGCAGAACAGTAGAAAGTGGAGCAGGGACAGGATGAAC AGTTTACCCCCCTCTTGCGAGAGGTATTGCCCATGCAGGAGCATCCGTAG ACTTAGCCATCTTCTCCTTACATCTAGCAGGTGTTTCATCAATTTTGGGA GCAGTAAATTTTATCTCAACAACAATTAACATAAAACCAAAAAATATAAA ACCCGAACGAATTCCACTATTCGTATGATCCGTCGCCATCACAGCTCTAC TCCTACTATTATCCCTGCCAGTACTAGCCGGAGCAATCACTATGTTATTA ACCGACCGCAACCTAAATACATCATTCTTTGACCCAGCTGGGGGTGGAGA CCCAATCCTATATCAACACTTATTCTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
Report Inappropriate Post