MAC-DHP, Orthoptera

April 14, 2025

Sample information
Picture
Location
Collection date 01/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Time of collection: 1:00-1:30
  • location of collection: A pond and small stream
  • Description of the environment: A pond and a small stream
  • Method of collection: dip net and forceps
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I believe my confidence level is medium because if we did not get a positive marks of BP for Wolbachia, there are are faint marks that indicate that there may be either a positive or lab error.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
Report Inappropriate Post