Long Horned Bee

Sample information

Picture
Photos by: Nicole D
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

The picture attached above was taken after death under a dissection scope, because no picture could be taken at the time of sample collection. It was found under a rock in Red Clay soil. It was identified using a Bug Guide as a Long Horned Bee under the genus Melissodes.

Putative identification Arthropoda Insecta Hymenoptera

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

When the gel was analyzed, there were no visible bands on the first lane, where the DNA Standard Ladder was inserted. So the gel was contaminated, or ran incorrectly, giving us completely inconclusive results. This gel was also running a reaction that tested for arthropod DNA, and a Wolbachia test gel did not get run.

Results

Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

Our gel was inconclusive, and the positive controls did not work, so I can have high confidence that the sample is unknown for Wolbachia. Our gel could have bene inconclusive because of problems when eluting the sample DNA, or when conducting the PCR to amplify the DNA.

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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