ZET-AGW

April 15, 2025

Sample information
Picture
Photos by: Audrey W
Location
Collection date 01/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected 1/28/2025 at 10:28 AM.

Next to the Department of Biology Greenhouse on the GSU Armstrong Campus.

Cold, Wet, Muddy, Rocky collection site.

Collected using forceps.
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All protocols were taken correctly and carefully followed to get these results.
Wolbachia 16S sequence
Arthropod COI sequence
GAGCAGGTATAGTTGGAACATCCATAAGTATTTTAATTCGAACTGAACTa gcactaCCAGGATCACTAATCGGAGATGATCAAATTTATAATGTAATCGT AACAGCTCATGCTTTTGTTATAATTTTCTTTATAGTTATACCTATTATAA TTGGGGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGAT ATGGCATTCCCTCGAATAAATAATATAAGATTTTGATTACTTCCCCCTTC TCTTACTATACTACTTGCTAGAAGTATAGTTGATATAGGTGCAGGAACTG GGTGAACTGTTTATCCCCCCCTTTCTGCAGGAATTGCCCATGCTGGGTCA TCTGTAGATATGGCTATTTTTTCATTACACCTTGCAGGAGTTTCCTCAAT TCTAGGAGCTGTAAACTTTATTACCACAATAATTAATATACGTGCTCCAG GAATATCTCTTGATCAAACACCTTTATTTGTATGAGCTGTAGGAATTACA GCTCTTCTTCTCCTCCTATCTCTCCCCGTTCTGGCGGGAGCAATTACTAT ACTATTAACTGACCGAAACTTAAATACTTCTTTCTTTGATCCTGCTGGAG GAGGAGATCCAATTCTTTATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
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