Califiera

April 15, 2025

Sample information
Picture
Photos by: Haroon M.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students
Putative identification Arthropoda Hexapoda Insecta Orthoptera Acrididae Melanoplus Melanoplus differentialis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Not 100% sure if gel electrophoresis was done accurately.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
CGGAAGGAACGTATCTGGTATTATTGATACCAACATCCTGCAGGATCAAAGAATGATGTATTTAAGTTTCGATCAGTTAATAATATTGTAATAGCTCCTGCTAGTACAGGAAGTGATAATAATAAAAGAAGTGCTGTAATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAATGTTATTCTTTCTGATCGTATATTGATTGCTGTTGTAATAAAATTAACTGCTCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAGAAAATGGCTAAATCAACTGATGCTCCTCCATGTGCAATTGCTCCAGCGAGCGGAGGGTAAACTGTTCATCCTGTACCAGCTCCTCTATCAATTATGGATGAAGGAAAGAAAAAAGGGTTAAGTGAGGGGGGAAAAAGTCAAAAACTTTTTTTTATTTTATTCGGGGGAAATGCTATTTCTGGTGCTCCAATTATTAATGGTACAAGTCAATTTCCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAATGCGTGGGCTGTAATAATTACATTATAGATTTGGTCATCTCCAATTAAAGATCCTGGTTGACCTAATTCTGCTCGAATAATTATTCTTATAGAAGTTCCTACTATTCCAGCTCATGCTCCAAATATAAAGTATAAGGTACCAATATCTTTATGATTTGTTGACCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Melanoplus differentialis was found to be negative for Wolbachia.
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