April 15, 2025

Sample information
Picture
Photos by: Amalia B
Location No map location data available.
Collection date 02/04/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Ant hill in dry, dead, grass environment.

Collection Tray, picked up with hand.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am 99% confident that my insect does not have Wolbachia. There was nothing during the gel electrophoresis results that gave me any doubt.

GCAGGAATAATTGGATCATCTATAAGAATAATCATCCGATTAGAACTAGGCTTTTGTGATTCTTAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACACGCTTTTATTATAATTTTTTTTATAATTATGCCCTTCATAATTGGAGGATTTGGAAATTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCTCGAATAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTTACTTATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCTACCCACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTATCTATTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTATTAATTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAGATAAAATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTTTTACTTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGACCGTAATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCATTCTATACCAACATTTATTCTGATTTTTTGGTC
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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