MAC-DLR

April 15, 2025

Sample information
Picture
Photos by: Dakota R.
Location
Collection date 01/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

cold day, sun out, grassy field
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

felt confident everything was done correctly.
Wolbachia 16S sequence
Arthropod COI sequence
CTAATTAATAATGATCAAATCTACAACTCctatatctttAtgatttgTTg accaaTAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTGGAA ATTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCTCGA ATAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTTACT TATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCTACC CACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTATCT ATTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTATTAA TTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAGATA AAATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTTTTA CTTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGACCG TAATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCATTC TATACCAACATTTATTCTGATTTTTTGGTCACCCTGGAAGTTTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
Report Inappropriate Post