MAC-SMW Coleoptera

April 15, 2025

Sample information
Picture
Photos by: Symon W.
Location No map location data available.
Collection date 01/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

My bug has three legs and three sections to the bugs body. The bug has a head, thorax, and abdomen. This bug has been caught on campus at Armstrong Georgia Southern in Savannah, GA. This bug was captured on a leaf of a tree.
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

Gel electrophoresisoresis shows a positive result for Wolbachia, but the BLAST shows that there is no Wolbachia present in my sample.
Wolbachia 16S sequence
Arthropod COI sequence
GTAGGAACATCGTTAAGTATTTTaanttcGGTTAGAATTagcatttAGAG GAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCAT GCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTT TGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTC CACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTT TTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGT TTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATT TAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCA GTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACT TGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTT TATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACT GACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCC AATTTTATACCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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