WRIKDM-16

April 16, 2025

Sample information
Picture
Photos by: Kelese M.
Location No map location data available.
Collection date 01/29/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught in muddy, grassy, bushy, and tree environment.
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Since the prevalence rate was only 25% for my order, I predicted my insect would NOT be infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
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