WRITNA-12-COLEOPTERA

April 17, 2025

Sample information
Picture
Photos by: Tran, A.
Location
Collection date 01/29/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Habitat Description:A large enclosed green house with high humidity and moisture.
  • Collection Method:Sweep nets, jars, and hand collection.
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I was confident in my Wolbachia-negative result because Sample S1 showed no 708 bp band on the gel, consistent with a negative outcome. Additionally, previous data shows only 22% of Coleoptera are typically infected, which supports the likelihood of a negative result.
Wolbachia 16S sequence
Arthropod COI sequence
GTGGGAACATCCCTAAGAATACTAATTCGTACAGAATTaggcaatccTGG ATCTCTAATTGGAGACGACCAAATTTATAACGTactTgtAACAGCACATG CTTTTATTATAATTTTCTTTATAGTTATACCAATAATAATCGGGGGATTC GGAAATTGATTAGTCCCCCTCATACTAGGAGCACCTGACATAGCATTCCC ACGATTAAATAATATAAGATTCTGGCTTCTTCCACCATCATTAACTCTTC TTCTAATAAGAAGAATCATTGACAAAGGTGCAGGGACAGGTTGAACTGTT TACCCACCCTTATCAGCTAATGTGGCACATGAAGGACCTTCTGTAGACTT GGCTATTTTCAGACTCCATATAGCTGGTATTTCCTCAATTTTAGGGGCCA TTAATTTTATCTCTACAGTTATAAATATGCGACCTAAGGGTATAACCCTT GACCGTATACCACTTTTTGTATGAGCAGTTAAAATTACTGCTATTCTCCT TCTTCTATCCTTACCTGTATTAGCAGGAGCTATCACTATGCTACTAACAG ACCGTAATATCAACACTTCCTTCTTTGACCCCGCAGGAGGAGGTGATCCT ATCTTATACCAACACTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
Report Inappropriate Post