Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 01/30/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collection Date 1/30/2025 Collection Time 10:30 am Collection Method Aerial/Sweep Net Location of Collection Georgia Southern University Description of Habitat Dry grass, wet mud under |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Solenopsis |
Methods |
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| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | 15% of insects from Hymenoptera are infected with Wolbachia in this location. Data also suggests Wolbachia infection to be unlikely. Controls worked, few errors in results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
ATCATCTATAAGAATAATCATCCGATTAGAACTAggctctTgtagtTCTC TAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACACGCTTTT ATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTGGAAA TTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCTCGAA TAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTTACTT ATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCTACCC ACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTATCTA TTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTATTAAT TTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAGATAA AATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTTTTAC TTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGACCGT AATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCATTCT ATACCAACATTTATTCTGATTTTTTGGTCA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Solenopsis was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid