BRO-KAM-Hemiptera

April 17, 2025

Sample information
Picture
Location
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Black and red-orange insect by a tree.
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location savannah ga
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I was confident that my insect wasn’t going to be infected because of a previous local study.
Wolbachia 16S sequence
GacCCGAGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGAT TCcaacTttaTGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCT TTTAAGGGATTAGCTTAGCCTTGCGACTTTGCAGCCCATTGTAGCCACCA TTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATC ATCCCCACCTTCCTCCAGTTTATCACTAGCAGTTTCCTTAAAGTCCCCAG CATTACCTGATGGTAACTAAGGATGAGGGTTACGCTCGTTGCGGGACTTA ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTG AAATCCGGCCGAACCGACCCTATCCCTTCGAATAGGTATG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TGATCAGGTATAGTTGGGTCATCCTTAAGATGAATTATTCGAATTGAACt ggccctgcgagGTTCATTTATTGGAGATGATCAAATTTATAATGtcatTG TAACTGCTCATGCATTTATTATAATTTTCTTTATAGTTATGCCAATTATA ATTGGAGGTTTTGGTAATTGGCTAGTACCTTTAATAATTGGTGCACCAGA TATAGCATTCCCCCGAATAAATAATATAAGTTTTTGGTTATTACCCCCAT CATTAACCCTACTATTAAGAAGTAGCATGGTTGAAATAGGGGCAGGAACA GGTTGAACTGTTTATCCTCCATTATCAAGCAATATTTCACATAGAGGGGC TTCAGTAGATTTAGCTATTTTTTCCCTCCATTTAGCAGGAGTTTCATCTA TTTTAGGAGCAATTAATTTTATCTCAACTATTATAAATATACGCCCAGCA GGTATAATTCCTGAGCGAACTCCTCTCTTTGTATGATCAGTGGGAATTAC TGCTCTTCTATTACTTTTATCCTTACCTGTTTTAGCAGGGGCTATCACTA TATTATTAACTGATCGTAATTTTAATACTTCCTTTTTTGACCCCACTGGT GGAGGAGACCCTATCCTTTATCAACACTTATTTTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hexapoda was found to be postive for Wolbachia.
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