GRE-KLJ Order Blattodea

April 17, 2025

Sample information
Picture
Photos by: Kaylanna J
Location
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident that my insect tested positive for Wolbachia as there was no errors made during the Gel Electrophoresis process.
Wolbachia 16S sequence
CCGagtaCGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCC AACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTT AAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTG TAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATC CCCACCTTCCTCCAGTTTATCACTAGCAGTTTCCTTAAAGTCCCCAGCAT TACCTGATGGTAACTAAGGATGAGGGTTACGCTCGTTGCGGGACTTAACC CAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAA TCCGGCCGAACCGACCCTATCCCTTCGAATAGGTATG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TTTTGgtgaTGATCCGGAATagtntggaaaTCATTAAGAATATTAATTCG TGCAGAATTaactcatcCTGGATCATTAATTGGAGATGATCAAATTTATA ATGTTATTGTAACAGCTCATGCCTTTGTTATAATTTTTTTTATAGTTATA CCTATTCTTATTGGAGGGTTTGGTAATTGATTAGTACCTTTAATACTAGG AGCTCCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGATTAC TTCCTCCTTCTTTAACTTTATTATTAGCGAGCAGTATAGTTGAAAGTGGT GCTGGAACAGGTTGAACTGTATACCCACCACTTGCAAGTGGTATTGCTCA TGCAGGAGCTTCTGTAGACTTAGCTATTTTCTCTTTACATTTAGCAGGTG TATCTTCAATTTTGGGAGCAGTTAACTTTATTTCAACAACAATTAATATA AAACCAATTAATATAAAGCCAGAACGAATTCCTTTATTTGTTTGAGCTGT AGGAATTACAGCATTATTGTTATTATTATCATTGCCTGTACTAGCAGGAG CAATTACAATATTACTTACAGATCGTAATTTAAATACTTCATTTTTTGAT CCCGCCGGTGGTGGAGATCCAATTCTTTATCAACATTTATTCTGATTTTT TGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be postive for Wolbachia.
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