GRE-MNR

April 17, 2025

Sample information
Picture
Photos by: Image provided by Mason N. Riegner, and used with permission
Location No map location data available.
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

mall insect, maroon brownish color
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 0.25 TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

This was my first time my results could have been batter.
Wolbachia 16S sequence
Arthropod COI sequence
ATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTaggtatttcaga tTCATTAATTAATAATGATCAAGTTTATAATTCTAtagtTACTAGACATG CTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTT GGAAATTTTTTAGTTCCTTTAATACTAGGATCCCCAGATATAGCCTATCC TCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTAC TTTTATTAAGAAATTTCATTAATGATGGAGTGGGAACAGGATGAACTGTT TACCCTCCATTAGCTTCTAATATTTTTCATAATGGCCCTTCAGTTGACTT AACAATTTTTTCTCTTCATATTGCGGGAATATCTTCTATTCTAGGAGCTA TCAATTTTATTTCAACAATTTTAAATATACGACATAAAAATTTTTCTTCT GATAAAATTCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACT TCTTCTTTCTTTACCAGTTCTTGCTGGAGCTATTACTATACTTTTAACTG ACCGAAATCTTAATACTTCCTTTTTTGATCCCTCAGGCGGAGGAGACCCT GTTCTTTATCAACATTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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