Sample information
Picture
Location
Collection date	11/04/2030
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected by Fall 2024 students
Putative identification	Arthropoda Insecta Diptera Muscidae Coenosia Coenosia tigrina
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	In front of University Building
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence	Download AB1
Arthropod COI sequence
Summary	The Coenosia tigrina was found to be negative for Wolbachia.