GRE-LCH Coleoptera

April 17, 2025

Sample information
Picture
Photos by: Lillie H.
Location No map location data available.
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

head, thorax, abdomen, mandibles, 2 sets of wings, no pinchers
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location head and lower body
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my results were accurate to my prediction
Wolbachia 16S sequence
Arthropod COI sequence
GTGGGAACCTCATTAAGAATACTTATTCGAGCTGAACTacgtattcCTGG ATCATTAATTGGAGATGACCAAATTTATAATGTAATTGTTACTGCTCATG CATTTATTATAATTTTTTTTATAGTTATACCAATTTTAATTGGGGGATTC GGAAATTGACTTGTACCTTTAATATTAGGAGCCCCTGATATGGCTTTCCC TCGAATAAATAATATAAGATTTTGATTACTCCCTCCATCACTGACTCTTT TACTGATAAGAAGCATAGTTGAAAGTGGGGCTGGTACAGGTTGAACTGTC TACCCCCCACTGTCATCAGGAATTGCTCATAGAGGAGCCTCAGTTGATTT AGCTATTTTTAGTCTTCACCTTGCAGGAGTTTCATCTATTCTAGGAGCTG TGAATTTTATTACAACAATTATTAATATACGTTCTACAGGAATAACATTT GAACGAATACCCCTATTTGTATGATCAGTAGGAATTACAGCTCTTTTACT TCTTTTATCTTTACCAGTATTGGCTGGAGCTATTACTATATTACTTACAG ATCGGAATTTAAATACCTCATTCTTTGACCCCGCAGGAGGAGGAGACCCT ATTTTATATCAACACTTATTCTGATTTTTTGGTCACCCTGAAGTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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