Cryptocephalus – E2

April 8, 2021

Sample information
Picture
Photos by: Eugen F
Location
Collection date 03/18/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

We found it unde ra tree bark
Putative identification Arthropoda Hexapoda Insecta Coleoptera Chrysomelidae Cryptocephalus

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I followed all the orders.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Cryptocephalus was found to be negative for Wolbachia.
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