Sample information |
|
Picture |
|
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Location | |
Collection date | 03/18/2021 |
Captive / Cultivated? | Wild-caught |
Group | KantiWil |
Observations | We found it unde ra tree bark |
Putative identification | Arthropoda Hexapoda Insecta Coleoptera Chrysomelidae Cryptocephalus |
Methods |
|
Extraction kit | Edwards Buffer |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Fast Blast |
Gel images |
|
Protocol notes | |
Results |
|
Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | I followed all the orders. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Cryptocephalus was found to be negative for Wolbachia. |