Polistes fuscatus

April 21, 2025

Sample information
Picture
Photos by: Addison B
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students
Putative identification Arthropoda Insecta Hymenoptera Vespidae Polistinae Polistes Polistes fuscatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Had a band but not a super strong one and the BLAST match was less then 100% but above 99%
Wolbachia 16S sequence Download FASTA    Download AB1
TNNNNNNNNNNNNTNNNNNNNNANNNNNNNNNNNNNNNANGANTAATNNGNNNNnNNNNNNNNNNNNNNNNNNNNNNNNC ANNNCNNNNnNNCNNNNTAATNTTNTTNATNNTNNTNNNNNNNNTANNNNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNN NANNNNNANNNNNNNANATANNNNTNNNNNNNNNNNATNNCNTACCTANNNNNGGGATAGGGTCGGTTCGTCCGGGTTTC ACACAGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCT TAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTC ATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAA TCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGNGGATCA GCACGCCACGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTATATTTTNTCTTTGGTCTATGAGCTGGTATAATNGGAAGATCATTAAGATTAATTATTCGTCTAGAATTAGGTACACC TTTATCAATTATTAATAATGATCAAATCTATAATTCAATTATTACAGCTCANGCATTAATTATAATTTTTTTCATAGTTA TACCATTTATAATTGGAGGATTNGGAAATTGATTAATTCCCTTAATATTAGGTGCCCCAGATATAGCATTCCCACGAATA AATAATATAAGATTTTGATTATTACCACCATCATTAATATTATTAATTATTAGAAATATTAATGATATAGGAGTAGGAAC TGGATGAACTTTATATCCACCTTTATCATCTAATATAGGTCACAATTCATCATCAGTTGATTTTGCTATTTTCTCTCTTC ACATTGCAGGAATTTCATCAATTATAGGAGCTATTAATTTCATTGTAACAATCTTAAATATACATATTAAAACTCATTCA TTAAATTTTTTACCTATATTTACATGATCAGTTTTAATTACTGCAATTTTACTTTTATTATCTTTACCTGTTCTAGCNGG ANNNNNNTANNNNNNTATTAACTGATCGAAATANNAATNCNNNNNT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Polistes fuscatus was found to be postive for Wolbachia.
Report Inappropriate Post