Erythemis simplicicollis

April 22, 2025

Sample information
Picture
Photos by: Zoya A
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students
Putative identification Arthropoda Hexapoda Insecta Odonata Libellulidae Erythemis Erythemis simplicicollis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no Wolbachia band present at all in my gel results. The controls worked because we were able to see the W- control not having a band for Wolbachia while the W+ and DNA+ did.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
>68-A_AY3_Co1_R.scf (586 bp) GTATAGTAANNNNNNNNNNNNNNNACAGGNAGGNNNAAAAGTAATAATNNNNNNNGTANNNNNNNNNNNNNCATACAAAA AGAGGTATNNNNNCCANCTNNNTCCCAGGGGATTTTATATTGATTACNGTTGTAATAAAATTAATAGCTCCTAGAATGGA GGANACNCCTGCTAAGTGTAACGAGAAAATAGTTAAATCNACNNATGCTCCTGCATGTGCAATAGCTCCTGCCAATGGAG GATATACAGTTCATCCTGTTCCTGCTCCTCTTTCTACTATACTTCTAGATAAAAGTAATGTGAAGNNNNGAGGAAGTAAT CAAAAACTTATATTATTAAGACGGGGGAAGGCTATATCCGGGGCTCCTAATATTAAAGGAACTAACCAGTTTCCNAAGCC ACCAATTATAATAGGCATAACTATGNAGAANNTTATAACGAATGCGTGAGCAGTAACAATAACATTATAAATTTGATCAT CTCCNATTAAAGATCCTGGTTGGCCTAATTCNATTCNAATNNAAACTCTNAGAGCTGTTCNNATTATACCNGCCNNAGCA CCNAAGANTAAATACNAGGNTCCNAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Erythemis simplicicollis was found to be negative for Wolbachia.
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