Sample information
Picture
Location
Collection date	09/03/2025
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations
Putative identification	Arthropoda Insecta Hymenoptera Sphecidae Sphex Sphex pensylvanicus
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	My confidence is because my BLAST results were
Wolbachia 16S sequence	Download FASTA    Download AB1
Arthropod COI sequence	Download FASTA    Download AB1
Summary	The Sphex pensylvanicus was found to be negative for Wolbachia.