Neurocolpus nubilus

April 25, 2025

Sample information
Picture
Photos by: Alina K.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2024 students

Medium-sized true bug.  The body is elongate-oval and somewhat flattened. Color varies but is usually brownish to tan with some darker and lighter markings. Small head with long, thin antennae.

 
Putative identification Arthropoda Hexapoda Insecta Hemiptera Miridae Neurocolpus Neurocolpus nubilus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Even though my controls did not work as they were supposed for the gel electrophoresis. The control W+ 7th lane Wolbachia DNA and arthropod was DNA negative, expected bands for both. PCR may have failed due to pipetting error, missing reagent, or contamination Control DNA should have shown a band for the arthropod DNA, but only had a band for Wolbachia. The concentration of DNA may have been too low for arthropod primers to amplify, the band present, a had moderate-intensity.

However, my BLAST DNA sequence results also showed no presence of Wolbachia, which showed my arthropod was unaffected, which led to a high confidence level.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
TGTTAATAGTATTGTNATGNNTCCTGCTAGAAN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Neurocolpus nubilus was found to be negative for Wolbachia.
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