Armadillidium vulgare #2 GRP30

May 21, 2025

Sample information
Picture
Photos by: Kennedy I
Location
Collection date 05/02/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes

1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp)

2. DNA sample of Pholcus phalangioides

3. DNA sample of Pholcus phalangioides

4. DNA sample of Armadillidium vulgare

5. Duplex positive control

6. Duplex negative control

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

A band was present in the lane, but the + Arthopod Control did not produce a band, so we can not be confident.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be postive for Wolbachia.
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