Armadillidium vulgare GRP7 (1)

May 21, 2025

Sample information
Picture
Photos by: Jeyuk B.
Location
Collection date 05/05/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Black, has many legs, looks like pillbug
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain 100X Fast Blast
Gel images
Protocol notes

Armadillidium vulgare of interest is in the fourth lane from the left. PCR with Wolbachia 16S primers was performed, so the band indicates that the organism was positive for Wolbachia.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Controls worked well, band was very visible on gel.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
NNNNNNNNNNNNNNNNNNNNNNNGCAGGGGCTGTTGGAACTGCCCTTAGA ATAATTATTCGCACTGAATTAGGACAACCTGGAAGTTTAATTGGAGATGA CCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTT TTATAGTAATACCTATTATAATTGGTGGATTTGGTAATTGGTTAATTCCA TTAATACTAGGAGCCCCAGATATGGCTTTTCCACGAATAAATAATATAAG ATTTTGACTTTTACCCCCTTCTTTAACTTTATTGCTTAGAAGTGGGTTGG TTGAAAGAGGAGTAGGAACAGGATGGACAGTATATCCTCCGCTGGCGTCT AGAATTGCTCATAGAGGAGCATCTGTAGATTTAGGTATTTTTTCTCTCCA CCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTGAATTTTATTACTACTA TAATTAATATACGAGCAGCTGGAATTAGAATAGACCGTGTTCCTTTATTT GTTTGATCAGTATTAGTAACGGCTGTACTTTTACTTTTATCCCTACCTGT ATTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCT CTTTTTTTGACCCTAGGGGAGGAGGAGACCCTATCCTTTATCAACACTTA TTTTGATTTTTTGGTCNNNTNGGAAGTTNNA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium vulgare was found to be postive for Wolbachia.
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