Wolbachia(-) Orthocladius sp.

June 4, 2025

Sample information
Picture
Photos by: Elijah M.
Location
Collection date 04/01/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Found within house very recently deceased.
Putative identification Arthropoda Insecta Diptera Chironomidae Orthocladius

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Lanes 3 and 7 are the Wolbachia and CO1 tests respectively.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked and bands showed up clear. The specimen was freshly dead and the BLAST result appears to match the photo of the organism.
Wolbachia 16S sequence
Arthropod COI sequence
TTTTGGAGCTTGATCAGGTATAGTAGGAACTTCTCTAAGAATTTTAATTCGAGCTGAATTAGGACATGCCGGTTCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCTCATGCTTTTGTTATAATTTTTTTTATAGTAATACCAATTTTAATTGGAGGATTCGGAAATTGATTAGTGCCTTTAATATTAGGTGCCCCTGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGATTATTACCCCCGTCATTGACTTTATTATTATCTAGTTCTATTGTAGAAAATGGAGCTGGGACAGGATGAACGGTTTACCCTCCTCTATCTTCTGGAATTGCTCATGCAGGCGCTTCAGTAGATTTAGCCATTTTTTCTTTACATTTAGCAGGAATTTCATCTATTTTAGGAGCTGTAAATTTTATTACAACAGTTATTAATATGCGGTCTAATGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAATTATTACTGCTATTTTATTGCTGCTATCTTTACCAGTATTAGCTGGAGCTATCACAATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCTGCTGGTGGAGGGGATCCTATTCTTTATCAACATTTATTCTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthocladius was found to be negative for Wolbachia.
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