Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/29/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | North Penn-Mansfield JSHS |
| Observations | Location – In riverside reeds, climbing on a blade of grass Notes – Appeared to possess wings, but did not attempt to fly away |
| Putative identification | Arthropoda Insecta Diptera Lonchopteridae Lonchoptera Lonchoptera bifurcata |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | All controls returned as expected, and the test lane has only one line in parallel with the negative arthropod control, however the line is smudged and unclear. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lonchoptera bifurcata was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica