Chrysoperla carnea (lace wing)

September 11, 2025

Sample information
Picture
Photos by: Bella H
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Four large, membranous, and highly translucent wings. Held roof like or tent like over the body at rest. Venation is intricate and delicate (“lace-like”).
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Cyber Stain
Gel images
Protocol notes
  1. IAH AK DNA strand
  2. IAH AK Arth. #1 Wol + Col1
  3. IAH AK Arth. #2 Wol + Col1
  4. IAH AK Wol Pos +Col1
  5. IAH AK Wol Neg +Col1
  6. IAH AK gDNA Control Wol+ Col1
  7. IAH AK water control Wol+Col1
  8. IAH AK Empty
  9. KG LR DNA strand
  10. KG LR Arth. #1 Wol + Col1
  11. KG LR Arth. #2 Wol + Col1
  12. KG LR Wol Pos +Col1
  13. KG LR Wol Neg +Col1
  14. KG LR gDNA Control Wol+ Col1
  15. KG LR water control Wol+Col1
  16. KG LR Empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Reviewing the outcomes of my lab test alongside the results from others who ran similar experiments has been really reassuring. This comparative look has given me a strong sense of confidence that my findings are not only valid but also correct.
Wolbachia 16S sequence
Arthropod COI sequence
NNNNNNNNNNNNNNNNCNNGNNNNNTNNNGNNNTGTCGNCNNCTCANGNNGNGTGANNTTGNGTAAGACCCCCNACCTNC GCGNTCTCTTCCTATGANGCATCANTTANNGCTGGTGACTTTCAGGAAACTGCNANAGAAAAACTGGNNNAANGTGGGGA TGATGTCNCGTNATCATGTGCNNTGANNNTGGGCTACTCACATGCTTGAGGGCTNNNNTNGNTATGCCNAACNNCNGTAA ANTTTTCTCATNAAAGCCTTCTCCNTCTTATTGGCCCTGCAACTCAATTGCATAAAGGTGGAATNNCTANTAATCGTGGA TCACCACCCCACNGNGAATACCTTCTCNNGTCTTGNACACCCNGACCNNTACGCCATGGGTAAAGGTCCNNNNTNNNNGT ATTANCTTTTTANNGNTNTCATTTTCTTTTNCTCAACTATTTGANTCTNNAACAAATTTAATNTNNANTTAATAACTTTA TNTGANNGACCAGAAGTTCTTATATCAGNNGNTTTATTATTAATGTTNCTAGCAATNGTTGNACCGATTNCGTACTAAAN NACAGATNTANATGATTCAACTTCNTNNNNTANNCAACCATTATGAGTCGTTGATCCTTNATCAACNTTATTAAAATTTT TGTCTTTTTNCATCTNTTNNNNNNNNGNNANNNNNNNNAAGNTTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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