Earwig

September 11, 2025

Sample information
Picture
Photos by: Aleena K
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Brown bug, hard shell/exterior, light brown limbs, pincers at rear abdomen.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Cyber Stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am highly confident that my arthropod is positive for Wolbachia because of the 2 bars that were visible on our gel.
Wolbachia 16S sequence
NNNNNNNNNNNNGGNGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCC TCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATG TCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCT AAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCG TGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
NNNNNNNNNNNNNNNNGNGNNTNNGNNNNNNAATAGTGGGGACTTCNTTNAGCTTGNTGATTCGGGCAGAANNNNNNNNN NNGGAGCTTTAATCGGAGATGATCAAATTTATAATNNNTNNGTTACGGCCCATGCTTTTGTAATGATTTTTTTTATGGTA ATGCCTATTATGATTGGAGGTTTTGGGAATTGACTGGTACCTTTGATGTTGAGAGCCCCAGATATGGCCTTTCCCCGAAT AAACAACATGAGTTTTTGGTTACTACCCCCTTCGCTTATGCTTTTGCTTTCTGGGAGTATGGTAGATAGAGGAGCTGGTA CAGGTTGGACAGTTTACCCCCCTTTGTCGGGGGCCATCGCCCACGCAGGGGCTTCGGTAGATTTGAGTATTTTTTCATTG CATTTGGCAGGGATTTCATCAATTTTGGGGGCAATTAATTTTATCACAACGGTGATTAATATGCGCCCATCGGGCCTTAA GCTGGAACGAATACCGTTGTTTGTATGATCCGTAGCCATTACTGCTCTTTTATTGTTGCTTTCTTTGCCAGTATTGGCAG GGGCTATCACTATGCTTTTGACGGACCGGAACTTAAATACATCTTTTTTCGACCCTGCGGGAGGGGGGGACCCCATTCTT TATCAACATTTATTTTGATTTTTTGGTCACCNNGGAAAGTTTNAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be postive for Wolbachia.
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