Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | Very aggressive and agitated when in captivity; would not stop buzzing. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | Cyber Stain |
| Gel images |
|
| Protocol notes | DNA extraction: The abdomen of the bee was crushed as finely as possible. There were some chunks of abdomen left in the sample however. 1.) DNA Standard 2.) Spider sample 3.) Bee sample 4.) Positive Wolbachia Control 5.) Negative Wolbachia 6.) Positive DNA Control 7.) Water (Empty)
|
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The bee sample (KKTS0302) was very close to what the positive DNA Control looked like. The controls worked fine. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica