Ant

Sample information

Picture
Location
Collection date 03/30/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Putative identification Hexapoda Insecta Hymenoptera Formicidae

Methods

Extraction kit Edwards Buffer
DNA extraction location Abdomen
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The pellet was dark, small, and barely visible.

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

A single PCR test for the first time using one is most definitely prone to faults occurring.

Arthropod control -A didn’t seem to work

-A, +A control in Wolbachia gels didn’t seem to work

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be negative for Wolbachia.
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