Sample information

Caught the arthropod by the base of a tree in a park. Caught it nearing the first day of fall in the end of the summer. The temperature when I caught it was 74* F in a terrestrial habitat. The arthropod was some kind of ant with antenna, and it was mainly black with some brown.

Methods

DNA Extraction:

Arthropod was not hard to extract DNA from. Crushed easily. No mistakes recorded.

PCR:

Had to redo the PCR because the first one didn’t work. Worked the second time around.

Gel electrophoresis, Arthropod CO1:

In the arthropod gel we found a band signaling that B-4 was indeed an arthropod. In the B1-4 lanes of the gel electrophoresis, we had the corresponding DNA for each arthropod. In the W+ lane was a Wolbachia positive control and in the W- lane was a Wolbachia negative control, and in the H20 lane was a water control. We also found bands for arthropods B1-3 and our W+ and W- controls. Our controls worked meaning that we are confident in the results. We had no mistakes in this process such as contamination.

Gel electrophoresis, Wolbachia:

There was a band for Wolbachia in the B-4 lane of our gel. In the B1-4 lanes of the gel electrophoresis, we had the corresponding DNA for each arthropod. In the W+ lane was a Wolbachia positive control and in the W- lane was a Wolbachia negative control, and in the H20 lane was a water control. We also found bands for B3 and our W+ control. There was no band for arthropods B1-2 and our W- control. Our controls worked as intended meaning that we can confidently say that B-4 was infected with Wolbachia.

Results

Our controls worked and we recorded no instances of contamination.