Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/17/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | On a tree near a building, 70 degrees, partly cloudy, blackish red color |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | Lanes 2 and 3 were my lanes for testing |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Camponotus pennsylvanicus was found to be negative for Wolbachia. |
European Paper Wasp
Woodworm Ant