Sample information
Picture	Photos by: Daelyn A.
Location
Collection date	10/15/2025
Captive / Cultivated?	Wild-caught
Group	Oak Ridge High School
Observations	small beetle, red stripe
Putative identification	Arthropoda Chilopoda
Methods
Extraction kit	pcr
DNA extraction location	Abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniPCR
Buffer	lysis
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	There was possible room for error but the procedure was accurate
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Chilopoda was found to be negative for Wolbachia.