Wolbachia(+) Dropephylla linearis

November 7, 2025

Sample information
Picture
Entry by: Elijah M.
Location
Collection date 06/20/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Caught live crawling on gravel near campgrounds.
Putative identification Arthropoda Insecta Coleoptera Staphylinidae Dropephylla Dropephylla linearis

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results are third row. Top: CO1. Bottom: Wol. Bottom Wolbachia test is to faint to see in the picture but there is a line there.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

BLASTing the Wolbachia sequence didn’t match to anything. EDIT: Resequenced for Wolachia, this time it matched in BLAST.
Wolbachia 16S sequence
TGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGCACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGAAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
AGGGACATCCCTCAGTCTTTTAATTCGAGCTGAACTAGGGAATCCTGGCACTTTAATTGGAGATGACCAAATTTATAATGTTATTGTAACTGCCCATGCATTTATTATAATTTTTTTTATAGTAATACCCATTGTTATTGGAGGATTCGGTAACTGATTAGTTCCTCTAATACTCGGAGCACCAGATATAGCATTCCCACGAATAAACAATATAAGATTTTGACTACTACCACCTTCATTAACCCTTCTTTTAATAAGAAGAATAGTTGAAAGAGGAGCTGGAACAGGATGAACTGTGTACCCCCCACTTTCATCAAATATTGCCCACGGAGGAGCCTCAGTTGACTTAGCTATTTTCAGGCTTCATTTAGCAGGAATTTCATCAATTTTAGGGGCTGTAAATTTCATCACTACAGTTATTAATATACGATCCTCAGGAATATCATTTGATCGAATACCTTTATTTGTATGATCTGTAGCTATCACAGCCTTACTTTTACTCTTATCTTTACCCGTATTAGCAGGAGCCATTACTATATTACTTACAGATCGAAATTTAAATACAACCTTTTTTGACCCCGCAGGAGGAGGTGACCCTATCTTATACCAACATTTATTTTGATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Dropephylla linearis was found to be postive for Wolbachia.
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