Wolbachia(?) Nabis alternatus

November 7, 2025

Sample information
Picture
Photos by: Elijah M.
Location
Collection date 06/21/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Collected live near campground.
Putative identification Arthropoda Insecta Hemiptera Nabidae Nabis Nabis alternatus

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results are seventh row. Top: CO1. Bottom: Wol. Wolbachia bar is somewhat faint on the picture.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

BLAST result for Wobachia didn’t match.
Wolbachia 16S sequence
TTATTACNANNNNNNTNANTTNNNTTNNCNCNANAAATANNNNATTANGNATCTNNTNNNCCCCCCTCCCTAANTANTAGGAANCTCNTTAAAANAGAGGATCNNGACNGGGTGNGGCNTCCCCCCCCTNNTTCTTCNANNANNNGNGGNNCGAGNNCNNTNGTACTNCCTTCTATTNTCTCTTCCTNTCGCNANGGNATCNGNANAACANGNNTAGAACTNCCTTTTCTANAAGTTNATATANNAAAAGAANGGNAANNNCCTCCCATCNTTTTTAANNNNNNTNCGNCAGNTCNAGNNCCNATCTCCTNCCNNNNTGNGNNNNTAGCNGANTGTATTGTNACATNNCNTGANATATTGNNTNGNCTTGNTCCNCCGCGACTAANCNNCNCTATNTNNACCCCTCTTCGGCTTTTGNNCNCNTCGGTTTNANATCGCGAAT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TAGGAACATCACTTAGTTGACTTATTCGTATTGAATTAGGACACCCTGGATCCTTTATTGGAGATGATCAAATTTATAATGTAGTTGTTACTGCACATGCTTTCATTATAATTTTCTTCATAGTAATACCAATCATAATTGGGGGATTTGGTAACTGACTAGTACCATTAATAATTGGAGCCCCCGATATGGCATTTCCCCGTATAAATAATATAAGTTTTTGACTATTACCACCCTCAATTACTCTATTAATTATTAGTAGAATTGTAGAAAAGGGAGTAGGGACAGGATGAACAGTTTATCCCCCATTATCAGCAAATGTAGCCCATAACGGTGCATCTGTAGATCTAGCCATTTTCAGATTACATTTAGCAGGTGTTTCTTCCATCCTAGGAGCAGTAAATTTTATTTCAACAATCATTAATATACGACCCGTAGGTATAACGCCAGAACGAATCCCCTTATTTGTATGATCTGTAGGTATTACAGCCCTACTACTTTTACTATCACTACCTGTATTAGCAGGAGCAATTACTATACTACTTACTGATCGTAATTTTAATACATCATTCTTTGACCCTGCAGGGGGAGGAGATCCTATTCTATATCAACATCTATTCTGATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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