DEMMCN Dermaptera

November 10, 2025

Sample information
Picture
Photos by: Milena N.
Location No map location data available.
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Under pine straw
  • Collected with Sweep net
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls all worked as expected.
Wolbachia 16S sequence
Arthropod COI sequence
CTTGATCGGGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGAG TTAGGTCAGCCTGGATCTTTAATTGGAGATGATCAGATTTATAATGTTAT TGTTACTGCTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATTA TAATTGGAGGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCCA GATATAGCTTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTCC ATCTCTGATTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGGA CAGGTTGGACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGGG GCTTCAGTGGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTTC TATTTTGGGAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCAG AAGGTTTAAAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTATT ACTGCTTTATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATTAC GATATTATTAACTGATCGAAATTTAAATACATCTTTTTTTGATCCAGCCG GAGGCGGAGATCCTATTTTATATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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