Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/26/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | The specimen was collected from a wild environment near Georgia Southern University, Armstrong Campus, Savannah, GA. A photograph of the specimen was taken and uploaded. The specimen was observed in its natural habitat prior to collection. |
| Putative identification | Arthropoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster |
Methods |
|
| Extraction kit | |
| DNA extraction location | savannah ga |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | A DNA extraction kit using in-house reagents was used to isolate genomic DNA from the insect specimen. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The positive and negative controls worked as expected. The PCR amplification produced clear bands at the expected size, and sequencing results for the Wolbachia 16S region matched known Wolbachia sequences in the database. This indicates that the assay was successful and the results can be trusted. |
| Wolbachia 16S sequence |
GTGGCGTGCTGATCCACGATTACTAGCGatcccttcttcgtgCACTCGAGTTGCAGAGTACAATCCGAACTGAgaTggcTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGACGAACCGACCCTATCCCTTCGAATAGGT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
TGGAACATCTTTAAGAATTTTAATTCGAGCTGAATtattacATCCTGGAGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCAGCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Drosophila melanogaster was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid