DEM-CRM Hymenoptera

November 11, 2025

Sample information
Picture
Photos by: Charleigh M
Location
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Date of collection:
-08/26/25
Time of collection:
– 1:30-2:00
Location of collection:
– Lane Library, Georgia Southern university
Environment of collection site:
– Bushes and flowers in a sunny location
Method of collection:
-Ariel/ Sweep Net above flowers and bushes
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident in my results because based on my insect order it has a low percent chance of being infected with Wolbachia and the gel electrophoresis solidified my prediction.
Wolbachia 16S sequence
Arthropod COI sequence
AGGTTCATCTATAAGATTAATTATTCGTATAGAACTAGGTATagcagttt cgttaCTTAAAAATGAACAATTATTTAATTCAATCGTTACTTCACATGCA TTCGTAATAATTTTCTTTTTTATTATGCCTCTAATAATTGGAGGTTTTGG CAATTGATTAATTCCTCTAATATTGGGAGTACCTGATATAGCTTTCCCTC GATTAAATAATTTAAGATTTTGACTATTACCTCCCTCTCTTATTTTATTA ATTTTTTCTAACTTTGTAGGAAAAGGAATTGGGACAGGATGAACTATTTA TCCCCCACTATCTAATTCTATTTACCATACTGGTATAGCAGTAGATTTAT TAATTTATTCCCTACATTTAAGAGGAATTTCTTCAATTCTAGGCTCAATT AATTTTATTGTAACAATTTTTAAAATACATAATTCTAATATTAAAATACA TTTAGTAATATTATTTCCTTGATCTATTTATATTACTACAATTTTATTAT TACTTTCATTACCAGTTTTAGCTGGGGCAATTACTATACTTTTATTTGAT CGAAACTTTAATACTTCATTTTTTGATCCCTCAGGAGGAGGAGATCCAGT TTTATATCAACATTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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