DEM-TLW – Diperta

November 11, 2025

Sample information
Picture
Photos by: Talia W
Location No map location data available.
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident on my results based on the data from the gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
TAGGAACTTCCCTTAGTATTATTGTTCGAATAGAATTAGGTCATCCtgtg gCATTAATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCTCATGC TTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTG GAAATTGACTTGTGCCTTTAATATTAGGAGCCCCTGATATGGCTTTTCCC CGAATAAATAATATAAGATTTTGAATATTACCTCCCTCTTTAACATTATT GTTAGCAAGAAGAATAGTAGAAAATGGGGCAGGAACAGGATGAACAGTTT ATCCACCTCTATCTGCAGGAATTGCTCATGGAGGAGCTTCTGTAGATTTA GCTATTTTTTCATTACATTTAGCAGGTATTTCTTCAATTTTAGGAGCAGT AAATTTTATTACTACAGTTATTAATATACGATCAACAGGTATTACATTTG ATCGAATACCATTATTTGTTTGATCTGTAGTAATTACTGCTATTTTATTA TTACTTTCTCTTCCTGTATTAGCAGGGGCTATTACTATATTATTAACTGA TCGTAATTTAAATACATCTTTTTTTGATCCTGCTGGAGGAGGAGATCCAA TTTTATATCAACATTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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