DEM-SFM Lepidoptera

November 11, 2025

Sample information
Picture
Photos by: Sophia M
Location No map location data available.
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found perched on a flower near the Learning Commons. Caught with a sweep net.
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reageants was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Databases show that Lepidoptera do not commonly contain Wolbachia infected DNA.
Wolbachia 16S sequence
Arthropod COI sequence
GTAGGAACATCTCTTAGTATTTTAATTCGAATAGAATTAGGTAATCCCgt aTCATTAATTGGTGATGATCAAATTTATAATACTATTGTTACAGCTCATG CATTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTT GGTAATTGATTAGTTCCATTAATATTAGGAGCCCCAGATATAGCATTTCC TCGAATAAATAATATAAGATTTTGACTTCTCCCCCCGTCATTAATTTTAT TAATTTCTAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACTGTT TATCCACCTCTTTCATCTAATATTGCTCATGGAGGTTCATCTGTAGATTT AGCTATTTTTTCCTTACATTTAGCCGGAATTTCTTCAATTTTAGGGGCAA TTAACTTTATTACTACTATTATTAATATACGAATTAATAATATATCTTTT GATCAATTACCTTTATTTGTTTGAGCTGTAGGAATTACAGCACTTCTTTT ATTATTATCTCTTCCAGTTTTAGCTGGAGCTATTACTATACTTTTAACAG ATCGAAATTTAAATACATCATTTTTTGATCCTGCTGGAGGGGGAGACCCA ATTTTATATCAACATTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be negative for Wolbachia.
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