Sample information |
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| Picture |
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|---|---|
| Location | No map location data available. |
| Collection date | 08/27/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | time: 1:40pm Georgia Southern University Armstrong campus in the field near the greenhouse caught with aerial net |
| Putative identification | Arthropoda Insecta Orthoptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Since my DNA concentration number was extremely low meaning full of protein so the results can’t be fully trusted since it was contaminated. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GTAGGCACATCATTAAGTATTTTAATTCGAACAGAACTAGGACAACCAGGCTATTTAATTGGAGATGATCAAACTTATAATGTTATCGTAACCGCACATGCATTTATCATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTACCATTAATATTGGGGGCTCCAGATATAGCCTTCCCACGAATAAACAATATAAGATTTTGACTTCTACCCCCATCATTAACCCTTTTATTAACCAGAAGAATAGTTGAAAATGGTGCAGGAACAGGATGAACAGTATACCCACCTCTATCAACAGGAATCGCTCACGCGGGAGCATCCGTCGATTTAGCTATCTTCTCACTACATTTAGCAGGAATTTCCTCAATTCTAGGGGCTGTAAATTTTATCACTACCATAATTAATATACGAGCTCCAGGAATATCACTAGACCAAACACCATTATTTGTTTGAGCAGTCGGAATTACAGCTCTTCTATTATTATTATCCTTACCAGTTTTAGCTGGTGCTATTACAATATTGCTTACAGATCGAAATTTAAATACATCATTTTTTGATCCAGCGGGAGGAGGGGATCCAATCTTATATCAACATCTATTTTGATTTTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Orthoptera was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica